Search results for "Debaryomyces hansenii"
showing 10 items of 10 documents
Species disparity response to mutagenesis of marine yeasts for the potential production of biodiesel.
2019
Abstract Background Among the third-generation biodiesel feed stock, oleaginous marine yeasts are the least studied microorganisms for such purpose. Results Wild strains yeasts were isolated from various Tunisian marine sources including fish waste (Candida tenuis CtTun15, Debaryomyces hansenii DhTun2015, Trichosporon asahii TaTun15 and Yarrowia lipolytica YlTun15) and seawater (Rhodotorula mucilaginosa RmTun15). Following incubation with ethyl methanesulfonate (EMS: 75 mM) for various periods of time (T15, T30, T45, T60 min), the cell viability of these strains responded differentially according to yeast species. For instance, mutated CtTun15 did not survive after 30 min of EMS treatment; …
Exponential growth phase cells of the osmotolerant yeast Debaryomyces hansenii are extremely resistant to dehydration stress
2001
Abstract The osmotolerant yeast Debaryomyces hansenii is highly resistant to dehydration stress and this tolerance was more pronounced for cells taken from the exponential growth phase than from the stationary phase. Growth of D. hansenii in medium containing 10% (w v −1 ) NaCl, resulted in an additional increase in cellular resistance to dehydration, which was most marked for stationary phase cells. It is expected that further investigations of the mechanisms behind this exceptional dehydrational tolerance will reveal new approaches for improvement of the quality of dry yeast.
Selection of Debaryomyces hansenii isolates as starters in meat products based on phenotypic virulence factors, tolerance to abiotic stress condition…
2021
Debaryomyces hansenii is a yeast widely used in meat fermentations as starter for the purpose of improving the aromatic quality of the final product. However, it has not been the subject of an extensive study regarding phenotypic characteristics important for starter selection, such as the capacity to grow at abiotic stress conditions occurring during fermentation, the ability to generate desirable aromas and the absence of virulence traits in yeasts.
Dairy yeasts produce milk protein-derived antihypertensive hydrolysates
2013
Abstract The potential of 20 dairy yeast strains belonging to Debaryomyces hansenii , Kluyveromyces lactis and Kluyveromyces marxianus species was examined for the production of milk protein-derived antihypertensive hydrolysates. For this purpose yeast strains were grown in microbiological medium with casein or lactoferrin as sole nitrogen source, and the inhibitory effects of casein and lactoferrin hydrolysates (CSHs and LFHs) on angiotensin I-converting enzyme (ACE) activity were determined. Based on the ACE-inhibitory activity, four CSHs and five LFHs were selected, and permeate fractions with molecular masses lower than 3 kDa (pCSHs and pLFHs) were obtained. In vitro ACE-inhibitory pote…
The Potential of the Yeast Debaryomyces hansenii H525 to Degrade Biogenic Amines in Food
2015
Twenty-six yeasts from different genera were investigated for their ability to metabolize biogenic amines. About half of the yeast strains produced one or more different biogenic amines, but some strains of Debaryomyces hansenii and Yarrowia lipolytica were also able to degrade such compounds. The most effective strain D. hanseniii H525 metabolized a broad spectrum of biogenic amines by growing and resting cells. Degradation of biogenic amines by this yeast isolate could be attributed to a peroxisomal amine oxidase activity. Strain H525 may be useful as a starter culture to reduce biogenic amines in fermented food.
Four new Candida cretensis strains isolated from Spanish fermented sausages (chorizo): Taxonomic and phylogenetic implications
2008
Four yeast strains were isolated from Spanish traditional fermented sausages (chorizo) spoiled by gas production. Using the classical identification procedures, they were identified as Debaryomyces hansenii. However, they fermented galactose and did not produce positive results in Debaryomyces differential medium (DDM), a growth medium highly specific for this species. Phylogenetic analysis showed identical sequences for the D1/D2 domain of the 26S rRNA gene and almost identical sequences for the 5.8S-ITS region with those of the recently described yeast species Candida cretensis. This result was confirmed by sequencing the gene encoding actin of the type and the new strains. Candida creten…
Polyphasic identification of yeasts isolated from bark of cork oak during the manufacturing process of cork stoppers.
2003
A two-step protocol was used for the identification of 52 yeasts isolated from bark of cork oak at initial stages of the manufacturing process of cork stoppers. The first step in the identification was the separation of the isolates into groups by their physiological properties and RFLPs of the ITS-5.8S rRNA gene. The second step was the sequencing of the D1/D2 domains of the 26S rRNA gene of selected isolates representing the different groups. The results revealed a predominance of basidiomycetous yeasts (11 species), while only two species represented the ascomycetous yeasts. Among the basidiomycetous yeasts, members representing the species Rhodosporidium kratochvilovae and Rhodotorula n…
Role of yeasts in table olive production
2008
Table olives are a traditional fermented vegetable of the Mediterranean countries, but their production and consumption are now spread all around the world. Yeasts can play a double role in this food. They are present throughout the fermentative process and it is generally accepted that they can produce compounds with important organoleptic attributes determining the quality and flavour of the final product. However, yeasts can also be spoilage microorganisms in olive fermentation/storage and packing causing gas pockets, swollen containers, cloudy brines and off-flavours and off-odours. Candida boidinii, Debaryomyces hansenii, Pichia anomala, P. membranifaciens, Rhodotorula glutinis and Sac…
Dehydration of yeast: Changes in the intracellular content of Hsp70 family proteins
2008
Abstract Yeast is known to experience in natural and industrial conditions cycles of dehydration–rehydration. Several molecular mechanisms can be triggered in response to this and other environmental stressors and to rescue yeast cells of the cytotoxic effect. Since heat shock proteins constitute one of the most important systems of the response to stress we studied whether the pre-induced major stress protein, Hsp70, can cope with yeast cell drying. To induce Hsp70 expression the cells of two yeast species, Saccharomyces cerevisiae and Debaryomyces hansenii , were subjected to non-lethal heat shock. It was found that during yeast culture growth Hsp70 accumulation occurred at the exponentia…
Dairy Debaryomyces hansenii strains produce the antihypertensive casein-derived peptides LHLPLP and HLPLP
2015
The ability of dairy Debaryomyces hansenii, Kluyveromyces lactis and Kluyveromyces marxianus strains to release the casein-derived antihypertensive sequences RYLGY, AYFYPEL, LHLPLP, HLPLP, VPP and/or IPP was examined. Yeast strains were grown in medium with casein as sole nitrogen source and the yeast casein hydrolysates (CSHs) were analysed by HPLC-MS/MS to search for the six antihypertensive sequences. Only LHLPLP and HLPLP were identified in CSHs and exclusively in D. hansenii Dh1 and Dh14 hydrolysates in which both antihypertensive sequences represented approximately 6 (CSH Dh1) and 10% (CSH Dh14) of total peptide content. In addition, a complete analysis of selected CSHs by HPLC-MS/MS …